The results of experimental show the enrichment of natural helium isotope 3He by filtering at a temperature less than 2,1 K. The features of application the low-temperature rectification are considered for separation of helium isotopes. The method of selection and the results of the numerical evaluation of the optimal parameters of work the distillation column are presented. he choice of the degree of enrichment in related stages of technology of reception the 3He isotope out of commodity helium based on the minimum total energy costs of cryostatting is justified. The estimation of the required specific cooling capacity means cryostating of superfluid helium in the installation receipt 3He isotope from natural helium is performed.